Composite

Part:BBa_K1973035

Designed by: José Martín Gómez   Group: iGEM16_UPO-Sevilla   (2016-10-14)


Pm1-glpF

Pm1 (BBa_K1973013) is one variant of the promoter for the xylS/Pm expression system. It was obtained by site-directed mutagenesis PCR from the Pseudomonas putida plasmid pMPO52 in order to remove illegal restriction sites. The expression of this promoter is activated by XylS or XylS2 in the presence of benzoate and salicylate, respectively. In this construct, Pm1 controls the expression of glpF (BBa_K1973026), a glycerol transporter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 326
    Illegal NgoMIV site found at 526
    Illegal NgoMIV site found at 601
    Illegal NgoMIV site found at 658
    Illegal NgoMIV site found at 1072
    Illegal NgoMIV site found at 1165
    Illegal AgeI site found at 499
    Illegal AgeI site found at 1015
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None